Professor David Gally

The Roslin Institute

Research Interests

My main research focus is the regulation of expression of surface organelles produced by Escherichia coli and Salmonella serovars. The work studies how the many different factors such as fimbrial adhesins, flagella, capsules and secretion systems are co-ordinated during infection to prevent interference with each another and excessive immune stimulation. Through this understanding we are contributing to the development of vaccines by targeting these virulence factors.

Specific projects are based on E. coli strains associated with severe gastrointestinal disease (enterohaemorrhagic E. coli O157 and O26), urinary tract infections and more recently an interest in strains associated with newborn meningitis and chronic infections (mastitis and Crohn’s).

The research uses genetic manipulation of the bacteria to knock out genes and to alter regulation pathways. We like to image the bacteria at the single cell level and aim to study sequential expression of surface factors by individual bacteria using a combination of fluorescent protein fusions and immunodetection.

Selected Publications

  • Geoffrey Mainda, Nadejda Lupolova, Linda Sikakwa, Paul R Bessell, John B Muma, Deborah V Hoyle, Sean P McAteer, Kirsty Gibbs, Nicola J Williams, Samuel K Sheppard, Roberto M La Ragione, Guido Cordoni, Sally A Argyle, Sam Wagner, Margo E Chase-Topping, Timothy J Dallman, Mark P Stevens, Mark Bronsvoort, David L Gally. 2016. Phylogenomic approaches to determine the zoonotic potential of Shiga toxin-producing Escherichia coli (STEC) isolated from Zambian dairy cattle. Scientific Reports Vol: 6. More»
  • Romina Fernandez-brando, Nao Yamaguchi, Amin Tahoun, Sean P. Mcateer, Trudi Gillespie, Dai Wang, Sally A. Argyle, Marina S. Palermo, David L. Gally. 2016. Type III Secretion-Dependent Sensitivity of Escherichia coli O157 to Specific Ketolides. Antimicrobial Agents and Chemotherapy Vol: 60 Pages: 459–470. More»
  • Anne Holmes, Claire Jenkins, Mary Hanson, Mark Woolhouse, John Wain, Lesley Allison, Margo Chase-topping, David Gally, George Gunn, Richard Ellis, Kathie Grant, Liljana Petrovska, Neil Perry, Lisa Byrne, Philip Ashton, Tim Dallman. 2015. Applying phylogenomics to understand the emergence of Shiga Toxin producing Escherichia coli O157:H7 strains causing severe human disease in the United Kingdom.. Microbial Genomics. More»
  • Amin Tahoun, Kirsty Jensen, Yolanda Corripio-Miyar, Sean P. McAteer, Alexander Corbishley, Arvind Mahajan, Helen Brown, David Frew, Aude Aumeunier, David G E Smith, Tom N. McNeilly, Elizabeth J. Glass, David L. Gally. 2015. Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella. Veterinary Research Vol: 46. More»
  • Yannick Rossez, Eliza B. Wolfson, Ashleigh Holmes, David L. Gally, Nicola J. Holden. 2015. Bacterial Flagella: Twist and Stick, or Dodge across the Kingdoms. PLoS Pathogens Vol: 11 Pages: e1004483. More»
  • Jai J Tree, Sander Granneman, Sean P McAteer, David Tollervey, David L Gally. 2014. Identification of Bacteriophage-Encoded Anti-sRNAs in Pathogenic Escherichia coli. Molecular Cell Vol: 55 Pages: 199-213. More»
  • A Lengeling, A Mahajan, D L Gally. 2013. Bacteriophages as pathogens and immune modulators?. mBio Vol: 4. More»
  • Louise Matthews, Richard Reeve, David L Gally, Christopher Low, Mark E J Woolhouse, Sean P McAteer, Mary E Locking, Margo E Chase-Topping, Daniel T Haydon, Lesley J Allison, Mary Hanson, George J Gunn, Stuart W J Reid. 2013. Predicting the public health benefit of vaccinating cattle against Escherichia coli O157. Proceedings of the National Academy of Sciences Vol: 110 Pages: 16265-16270. More»
  • Amin Tahoun, Simmi Mahajan, Edith Paxton, Georg Malterer, David S Donaldson, Dai Wang, Alwyn Tan, Trudi L Gillespie, Marie O'Shea, Andrew J Roe, Darren J Shaw, David L Gally, Andreas Lengeling, Neil A Mabbott, Juergen Haas, Arvind Mahajan. 2012. Salmonella transforms follicle-associated epithelial cells into M cells to promote intestinal invasion. Cell Host and Microbe Vol: 12 Pages: 645-56. More»